Product Description

BBL Prompt Incoculation System For use with the Disc Diffusion Susceptibility Test Intended Use: The BBL Prompt Inoculation System is used to prepare standardized suspensions of bacteria for the Bauer-Kirby disc diffusion antimicrobial susceptibility test procedure. The system may be used for rapidly growing bacteria such as Enterobacteriaceae, Staphylococcus spp., Pseudomonas aeruginosa, Acinetobacter spp., enterococci and some nonenterococcal streptococci. It also may be used for Haemophilus influenzae. Product Summary: The Bauer Kirby procedure is a standardized method for the determination of antimicrobial susceptibility based on the agar gel disc diffusion principle. This method is published as a consensus standard by the National Committee for Clinical Laboratory Standards (NCCLS) and is periodically reviewed and updated. The inoculum concentration has been shown to be an important factor in obtaining valid results with the Bauer Kirby procedure. The original Bauer- Kirby procedure employed inoculum prepared from log phase cultures. This requires incubation in broth for several hours to achieve the desired inoculum density, equivalent to the 0.5 McFarland turbidity standard, which produces approximately 1 x 108 CFU/mL. Several studies have subsequently shown that direct standardization of the inoculum, i.e., without incubation, is an acceptable alternative. The direct adjustment of inoculum preparation is the preferred method for fastidious organisms that may grow slowly in broth. The M2-T4 edition of the NCCLS disc diffusion standard contained a revised procedure for Haemophilus influenzae, in which a new medium, Haemophilus Test Medium (HTM), was employed. This procedure recommended matching the inoculum density to the 0.5 McFarland standard using a photometric device. The BBL Prompt Inoculation System is a device that allows direct standardization of inoculum without a The product referenced on this detail page is sold be 60/box.